Secretion of β-N-acetylglucosaminidase isoenzymes by normal human fibroblasts

Intraphagocytic Β-n-acetylglucosaminidase

The beta-N-acetylglucosaminidases of rabbit and human polymorphonuclear leukocytes and of rabbit alveolar macrophages have been studied in comparison with the beta-N-acetylglucosaminidase derived from a soil bacillus which had previously been shown to hydrolyze the group-specific polysaccharide of Group A streptococci. The phagocytic enzymes are lysosome associated and have an acid pH optimum. ...

Human ovarian carcinoma beta-N-acetylglucosaminidase isoenzymes and their role in extracellular matrix degradation.

Degradation and invasion of basement membrane by tumor cells involves the cooperative hydrolysis of proteoglycans, collagens, and glycoproteins mediated by a number of enzymes including proteases, collagenases, and glycosidases. In order to study these processes in vitro, a tissue culture system was developed in which bovine corneal endothelial cell extracellular matrix (ECM) serves as a substr...

Secretion of lysosomal enzymes by normal and cystic-fibrosis fibroblasts

The secretion of cellular components by mononuclear phagocytes, lymphocytes and neutrophils seems to play an important role in the inflammatory process (Davies & Allison 1976). It is likely that the secretory activity of one cell will be modified by the activities of the others. Since the macrophage is the predominant cell in chronic inflammation, we studied its lysosomal enzyme secretion and i...

Probing the Catalytic Mechanism of Vibrio harveyi GH20 β-N-Acetylglucosaminidase by Chemical Rescue

BACKGROUND Vibrio harveyi GH20 β-N-acetylglucosaminidase (VhGlcNAcase) is a chitinolytic enzyme responsible for the successive degradation of chitin fragments to GlcNAc monomers, activating the onset of the chitin catabolic cascade in marine Vibrios. METHODS Two invariant acidic pairs (Asp303-Asp304 and Asp437-Glu438) of VhGlcNAcase were mutated using a site-directed mutagenesis strategy. The...

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro